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S2Media™ Chocolate Agar

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S²Media™ prepared Chocolate Agar is an enriched, nonselective culture medium intended for the isolation and cultivation of fastidious microorganisms, particularly Haemophilus spp. and Neisseria spp. The medium combines a nutrient-rich GC agar base with hemoglobin and VitAmino™ Solution to provide essential nutrients, vitamins, cofactors, and growth factors required by nutritionally demanding organisms. The heated blood components provide readily available hemin (X factor), while the enriched formulation supports organisms requiring additional nutritional supplementation.


S²Media™ Chocolate Agar is formulated to provide performance comparable to Chocolate II™ Agar and is well suited for the primary cultivation and recovery of fastidious microorganisms from clinical specimens. Commercial Chocolate II™ Agar similarly uses GC II Agar Base, hemoglobin, and a defined enrichment supplement for cultivation of Haemophilus and Neisseria spp.


Also known as:

  • Chocolate Agar

  • Chocolate Blood Agar

  • GC Chocolate Agar

  • GC Agar with Hemoglobin and Enrichment

  • GC Agar with Hemoglobin and VitAmino™ Solution

  • Enriched Chocolate Agar

  • Heated Blood Agar


👉 Finished product meets the following standards and references:

  • CLSI


🧫 Application Areas:

  • Clinical microbiology for the cultivation of fastidious microorganisms

  • Isolation and cultivation of Haemophilus spp.

  • Isolation and cultivation of Neisseria spp.

  • Recovery of Neisseria gonorrhoeae and Neisseria meningitidis

  • Recovery of Haemophilus influenzae and other nutritionally demanding Haemophilus spp.

  • Respiratory specimen culture

  • Genital and urogenital specimen culture

  • Cerebrospinal fluid and other normally sterile body fluid culture

  • Primary cultivation of fastidious organisms from clinical specimens

  • Public health and reference laboratory microbiology

  • Microbiological quality control and culture maintenance applications


Equivalent or Comparable Item Numbers:

  • 221169

  • 221267

  • R01300

  • E14

  • E14BX

  • R01900

  • R01302

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