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S2Media™ BCYE Agar w/o L-cysteine

S2Media™ BCYE Agar w/o L-cysteine

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S²Media™ prepared BCYE Agar without L-cysteine is a differential control medium used for the presumptive confirmation of Legionella spp. isolated from water and environmental samples. Because Legionella spp. require L-cysteine for growth, typical colonies are subcultured in parallel to conventional BCYE Agar and BCYE Agar without L-Cysteine. Isolates that grow on BCYE but fail to grow on the cysteine-deficient medium are presumptively identified as Legionella. Based on Edelstein’s formulation and prepared in accordance with ISO 11731:2017, the medium supports confirmation of presumptive colonies recovered from selective media such as BCYE-GPVC, BCYE-PAC, or BCYE-PAV.


Also known as:

  • Buffered Charcoal Yeast Extract Agar without L-Cysteine

  • Buffered CYE Agar (CYE) without Cysteine

  • Legionella BCYE Agar without Cysteine


👉 Finished product meets the following standards and references:

  • CLSI

  • ISO

  • SM-WW


🧫 Application Areas:

  • Clinical microbiology for the selective isolation of Legionella spp.

  • Respiratory specimen testing for Legionella

  • Water microbiology and Legionella surveillance

  • Potable and non-potable water system testing

  • Building water management and monitoring programs

  • Cooling tower and HVAC-associated water testing

  • Healthcare facility environmental monitoring

  • Environmental investigation and contamination source tracking

  • Public health and epidemiological surveillance

  • Microbiological quality control and environmental testing programs


Equivalent or comparable item numbers:

  • 3563722

  • G108

  • 422230

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